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FTIR NIR Raman UV-Vis Mass Spec LIF Fluorescence PAT Process Spectroscopy
Registered in England 2919549
Spectroscopy from Lab to Line - since 1994
Clairet Scientific PAT spectroscopy
01604 494411
Light Induced Fluorescence as a PAT tool .
The most common spectroscopic techniques Clairet supplies for PAT are NIR and Raman, however, both of these techniques have a lower limit of quantitation of the order of a few hundred ppm (except in some cases where long pathlength NIR measurements are possible, on clear liquids for example.) Light Induced Fluorescence (LIF) can be a much more sensitive technique. Where the analyte of interest is fluorescent and needs to be measured in a matrix which is either non-fluorescing or where fluoresence is not excited by the same wavelength, LIF is ideal. The availability of LEDs of specific wavelengths in the UV or Visible provides a readily available monochromatic excitation source, more rugged and better suited to PAT than the conventional lamp and monochromator source arrangement used in laboratory instruments. Several LEDS can be installed in a single instrument to provide a range of selectable excitation wavelengths. A rugged monolithic diode array UV-Vis spectrograph is used for detection and analysis of the fluorescence signal. One example of the use of LIF is for monitoring the blending of low concentrations of active pharmaceutical ingredients (API's) with excipients. Caffeine provides a good model compound for illustrating this type of application. The NIR spectra of a series of low concentrations of caffeine are shown below: The excipients have strong NIR spectra, masking the changes in caffeine concentrations and limiting the sensitivity and accuracy of quantitation for an NIR method However, caffeine fluoresces strongly under UV excitation while the excipients do not, producing a strong clear signal for analysis at concentrations as low as 0.05%, shown below: The single peak in the fluorescence spectrum due to caffeine, with no interference from other components is ideally suited for univariate quantitative or qualitative analysis. Whilst not so universally applicable as NIR and Raman, LIF is a very useful addition to the PAT spectroscopy toolbox. LIF systems are manufactured by Prozess Technology and available from Clairet Scientific. They can be configured either with a probe or with a large area head designed to measure through a window (shown below). A variety of enclosures for lab or process applications including IP65 and ATEX are available. For rotating bin blender applications optional battery powered operation and wireless communications are available
NIR spectra of common pharmaceutical excipients plus low concentrations of caffiene Light induced fluorescence spectra of variuos concentrations of caffeine blended with excipients PAT measuring head for fluorescence, equipment for blend monitoring with LIF
The most common spectroscopic techniques Clairet supplies for PAT are NIR and Raman, however, both of these techniques have a lower limit of quantitation of the order of a few hundred ppm (except in some cases where long pathlength NIR measurements are possible, on clear liquids for example.) Light Induced Fluorescence (LIF) can be a much more sensitive technique. Where the analyte of interest is fluorescent and needs to be measured in a matrix which is either non-fluorescing or where fluoresence is not excited by the same wavelength, LIF is ideal. The availability of LEDs of specific wavelengths in the UV or Visible provides a readily available monochromatic excitation source, more rugged and better suited to PAT than the conventional lamp and monochromator source arrangement used in laboratory instruments. Several LEDS can be installed in a single instrument to provide a range of selectable excitation wavelengths. A rugged monolithic diode array UV-Vis spectrograph is used for detection and analysis of the fluorescence signal. One example of the use of LIF is for monitoring the blending of low concentrations of active pharmaceutical ingredients (API's) with excipients. Caffeine provides a good model compound for illustrating this type of application. The NIR spectra of a series of low concentrations of caffeine are shown below: The excipients have strong NIR spectra, masking the changes in caffeine concentrations and limiting the sensitivity and accuracy of quantitation for an NIR method However, caffeine fluoresces strongly under UV excitation while the excipients do not, producing a strong clear signal for analysis at concentrations as low as 0.05%, shown below: The single peak in the fluorescence spectrum due to caffeine, with no interference from other components is ideally suited for univariate quantitative or qualitative analysis. Whilst not so universally applicable as NIR and Raman, LIF is a very useful addition to the PAT spectroscopy toolbox. LIF systems are manufactured by Prozess Technology and available from Clairet Scientific. They can be configured either with a probe or with a large area head designed to measure through a window (shown below). A variety of enclosures for lab or process applications including IP65 and ATEX are available. For rotating bin blender applications optional battery powered operation and wireless communications are available
Light Induced Fluorescence as a PAT tool
Registeed in England 2919549 
Clairet Scientific on-line spectroscopy